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Targeted seed EMS mutagenesis reveals a basic helix-loop-helix transcription factor underlying male sterility in sorghum.

Genetics - 9 Jul 2025

Xiao Yuguo, Khangura Rajdeep S, Wang Zhonghui, Dilkes Brian P, Eveland Andrea L

Abstract excerpt

Forward genetic screens of mutant populations are fundamental for functional genomics studies. However, isolating independent mutant alleles to molecularly identify causal genes is challenging in species recalcitrant to genetic manipulation. Here, we demonstrate that classic seed ethyl methanesulfonate (EMS) mutagenesis coupled with genome sequencing can overcome this limitation in sorghum. We used this method to...

Topics

Sorghum
Plant Infertility
Seeds
Mutagenesis
Ethyl Methanesulfonate
Basic Helix-Loop-Helix Proteins
Plant Proteins
Phenotype
Mutation
Alleles

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PMID: 39882980
DOI: 10.1093/genetics/iyaf017
PMCID: PMC12239208

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