Article
Maize-targeted mutagenesis: A knockout resource for maize.
Proceedings of the National Academy of Sciences of the United States of America - 30 Sept 2003
May Bruce P, Liu Hong, Vollbrecht Erik, Senior Lynn, Rabinowicz Pablo D, Roh Donna, Pan Xiaokang, Stein Lincoln, Freeling Mike, Alexander Danny, Martienssen Rob
Abstract excerpt
We describe an efficient system for site-selected transposon mutagenesis in maize. A total of 43,776 F1 plants were generated by using Robertson's Mutator (Mu) pollen parents and self-pollinated to establish a library of transposon-mutagenized seed. The frequency of new seed mutants was between 10-4 and 10-5 per F1 plant. As a service to the maize community, maize-targeted mutagenesis selects insertions in genes...
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