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Ethyl methanesulfonate (EMS) mutagenesis with cucumber pollens and a case study of the map-based gene cloning with EMS-induced mutants.

Plant science : an international journal of experimental plant biology - 1 Mar 2026

Xiang Yachen, Cui Haibing, Fu Linglong, Wang Xiaoxue, Zhou Yuqing, Yao Senyang, Li Mimi, Liu Hanqiang, Pan Yupeng

Abstract excerpt

Ethyl methanesulfonate (EMS) mutagenesis is a widely employed strategy for inducing genetic variation in plants. While EMS mutagenesis of seeds has been utilized in cucumber, the potential of pollen-based mutagenesis remains largely unexplored in this important crop. To address this gap, we developed the first optimized pollen-EMS mutagenesis protocol for cucumber using the inbred line Poinsett 76. This approach...

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Chromosome Mapping
Cloning, Molecular
Cucumis sativus
Ethyl Methanesulfonate
Genes, Plant

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 41519239
DOI: 10.1016/j.plantsci.2026.112985

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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