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Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon

5 Oct 2013

Abstract excerpt

A method for rapid production of single-round infectious particles (SRIPs) of flavivirus would be useful for viral mutagenesis studies. Here, we established a DNA-based production system for SRIPs of flavivirus. We constructed a Japanese encephalitis virus (JEV) subgenomic replicon plasmid, which lacked the C-prM-E (capsid-pre-membrane-envelope) coding region, under the control of the cytomegalovirus promoter....

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PMID: 24096319
DOI: 10.1099/vir.0.058008-0

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