Label-free single-cell phenotyping to determine tumor cell heterogeneity in pancreatic cancer in real time.

doi:10.1172/jci.insight.169105

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Article

Label-free single-cell phenotyping to determine tumor cell heterogeneity in pancreatic cancer in real time.

JCI insight - 8 Jul 2025

Wittenzellner Katja, Lengl Manuel, Röhrl Stefan, Maurer Carlo, Klenk Christian, Papargyriou Aristeidis, Schmidleitner Laura, Kabella Nicole, Shastri Akul, Fresacher David E, Harb Farid, Hafez Nawal, Bärthel Stefanie, Lucarelli Daniele, Escorial-Iriarte Carmen, Orben Felix, Öllinger Rupert, Emken Ellen, Fricke Lisa, Madej Joanna, Wustrow Patrick, Demir I Ekin, Friess Helmut, Lahmer Tobias, Schmid Roland M, Rad Roland, Schneider Günter, Kuster Bernhard, Saur Dieter, Hayden Oliver, Diepold Klaus, Reichert Maximilian

Abstract excerpt

Resistance to chemotherapy of pancreatic ductal adenocarcinoma (PDAC) is largely driven by intratumoral heterogeneity (ITH) due to tumor cell plasticity and clonal diversity. To develop alternative strategies to overcome this defined mechanism of resistance, tools to monitor and quantify ITH in a rapid and scalable fashion are needed urgently. Here, we employed label-free digital holographic microscopy (DHM) to...

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Topics

Pancreatic Neoplasms
Single-Cell Analysis
Humans

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Animals

Mice

Carcinoma, Pancreatic Ductal

Phenotype

Holography

Cell Line, Tumor

Epithelial-Mesenchymal Transition

Machine Learning

Cell Differentiation

Source

Open in PubMed Open full text

Identifiers

PMID: 40424076
DOI: 10.1172/jci.insight.169105
PMCID: PMC12288891

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