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Article

Imaging oligomers of alpha-toxin (Hla) variants using high-speed AFM and neutralizing Hla hemolytic activity with their antisera.

Archives of biochemistry and biophysics - 1 Jun 2025

Le Ngan Thi Phuong, Ngo Kien Xuan, Nguyen Trinh Thi Ngoc, Tran Linh-Thuoc, Nguyen Hoang Duc

Abstract excerpt

Alpha-toxin (Hla) variants, such as the toxoids HlaH35A, HlaH35L, and HlaH35LH48L, have been shown to lack hemolytic activity and present promising antigen sources for vaccine development against S. aureus. The His35 site is critical in the oligomerization process of Hla during transmembrane pore formation, leading to cell lysis. This study employed high-speed atomic force microscopy (HS-AFM) to image the...

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Share to Antibodies, Neutralizing
Share to Bacterial Toxins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Hemolysin Proteins

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Share to Microscopy, Atomic Force

Share to Protein Multimerization

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Identifiers

PMID: 40147500
DOI: 10.1016/j.abb.2025.110403

Genes

AFM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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