The heptameric prepore of a staphylococcal alpha-hemolysin mutant in lipid bilayers imaged by atomic force microscopy.

doi:10.1021/bi970600j

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Article

The heptameric prepore of a staphylococcal alpha-hemolysin mutant in lipid bilayers imaged by atomic force microscopy.

Biochemistry - 5 Aug 1997

Fang Y, Cheley S, Bayley H, Yang J

Abstract excerpt

We have used atomic force microscopy to study the oligomeric state of a genetically engineered mutant of staphylococcal alpha-hemolysin (alphaHL-H5) that can be arrested as a "prepore" assembly intermediate. AFM images of alphaHL-H5 on supported bilayers of a fluid-phase lipid, egg-yolk phosphati...

Topics

Bacterial Toxins
Hemolysin Proteins
Lipid Bilayers
Microscopy, Atomic Force
Mutation
Protein Conformation
Recombinant Proteins

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