Article
Scalable genotyping of microbial colonies.
Microbial genomics - 1 Mar 2025
Chen Arnold, Nchinda Nkazi, Cira Nate J
Abstract excerpt
The sequence of the 16S region is taxonomically informative and widely used for genotyping microbes. While it is easy and inexpensive to genotype several isolates by Sanger sequencing the 16S region, this method becomes quite costly if scaled to many isolates. High-throughput sequencing provides one potential avenue for obtaining 16S sequences at scale but presents additional challenges. First, DNA purification...
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