Article
EV DNA from pancreatic cancer patient-derived cells harbors molecular, coding, non-coding signatures and mutational hotspots.
Communications biology - 5 Mar 2025
Olou Appolinaire A, Tom Wesley A, Krzyzanowski Gary, Jiang Chao, Chandel Dinesh S, Fernando Nirmalee, Draney Adrian W, Destino Joel, Welch Danny R, Fernando M Rohan
Abstract excerpt
DNA packaged into cancer cell-derived EV is not well appreciated. Here, we uncovered signatures of EV DNA secreted by pancreatic cancer cells. The cancer cells and non-cancer counterparts exhibit distinct low vs. high molecular weight (LMW vs. HMW) EV DNA fragments distribution, respectively. Genome sequencing and Single Nucleotide Variants analysis revealed that 95% of reads and 94% of SNVs map to noncoding...
Read the complete abstract on PubMedTopics
- Humans
- Pancreatic Neoplasms
- Mutation
- Cell Line, Tumor
- Polymorphism, Single Nucleotide