Article
Mutation detection using ENDO1: application to disease diagnostics in humans and TILLING and Eco-TILLING in plants.
BMC molecular biology - 23 Apr 2008
Triques Karine, Piednoir Elodie, Dalmais Marion, Schmidt Julien, Le Signor Christine, Sharkey Mark, Caboche Michel, Sturbois Bénédicte, Bendahmane Abdelhafid
Abstract excerpt
BACKGROUND: Most enzymatic mutation detection methods are based on the cleavage of heteroduplex DNA by a mismatch-specific endonuclease at mismatch sites and the analysis of the digestion product on a DNA sequencer. Important limitations of these methods are the availability of a mismatch-specific endonuclease, their sensitivity in detecting one allele in pool of DNA, the cost of the analysis and the ease by...
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