Article
Experimental Evolution of Poxviruses.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025
Banerjee Shefali, Jewell Kelsea A, Brennan Greg
Abstract excerpt
Experimental evolution is the process of exposing virus populations to defined selective pressures in a laboratory setting to identify adaptive changes. Coupled with deep sequencing, this experimental approach allows for nucleotide-level resolution of poxvirus adaptive strategies over time. Here, we present a general method of poxvirus experimental evolution, Illumina-based deep sequencing, and bioinformatic...
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