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Article

Dominant negative mutations in yeast Hsp90 indicate triage decision mechanism targeting client proteins for degradation.

Molecular biology of the cell - 1 Jan 2025

Flynn Julia M, Joyce Margot E, Bolon Daniel N A

Abstract excerpt

Dominant negative (DN) mutations provide valuable tools for investigating protein mechanisms but can be difficult to isolate because of their toxic effects. We used a mutational scanning approach to identify DN mutations in yeast Hsp90. In a previous mutational scan of the ATPase domain of Hsp90, we noticed that many mutations were at very low frequency after outgrowth in cells coexpressing wildtype Hsp90. Most...

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Identifiers

PMID: 39565679
DOI: 10.1091/mbc.E24-07-0309
PMCID: PMC11742116

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