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Metabolic Phenotyping of Synaptic Mitochondria Using MitoPlates™ and Synaptoneurosomes.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Stawikowska Aleksandra, Dziembowska Magdalena, Kuzniewska Bozena

Abstract excerpt

Mitochondrial functional assays using MitoPlates™ S-1 allow us to characterize mitochondria in terms of substrate metabolism. MitoPlates™ are 96-well microplates pre-coated with a diverse set of substrates. The electron flow from NADH and FADH2 producing mitochondrial substrates is measured based on the reduction of redox dye, that acts as a terminal electron acceptor. Here, we describe the application of...

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PMID: 39546257
DOI: 10.1007/978-1-0716-4264-1_4

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