Article
α-Amylase, glucoamylase and isopullulanase determine molecular weight of pullulan produced by Aureobasidium melanogenum P16.
International journal of biological macromolecules - 1 Oct 2018
Liu Nan-Nan, Chi Zhe, Liu Guang-Lei, Chen Tie-Jun, Jiang Hong, Hu Zhong, Chi Zhen-Ming
Abstract excerpt
A high molecular weight (Mw) pullulan has many potential applications in various fields. α-Amylase, glucoamylase and pullulanase were thought to play an important role in high Mw pullulan biosynthesis. However, there is no genetic evidence for this role. In this study, the genes encoding α-amylase, glucoamylase and pullulanase were cloned from Aureobasidium melanogenum P16, a high pullulan producing yeast and...
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