Article
Collapsed State Mediates the Low Fidelity of the DNA Polymerase β I260 Mutant.
Biochemistry - 1 Oct 2024
Fijen Carel, Chavira Cristian, Alnajjar Khadijeh, Sawyer Danielle L, Sweasy Joann B
Abstract excerpt
DNA polymerase β (Pol β) fills single nucleotide gaps during base excision repair. Deficiencies in Pol β can lead to increased mutagenesis and genomic instability in the cell, resulting in cancer. Our laboratory has previously shown that the I260 M somatic mutation of Pol β, which was first identified in prostate cancer, has reduced nucleotide discrimination in a sequence context-dependent manner. I260 M...
Topics
- DNA Polymerase beta
- Fluorescence Resonance Energy Transfer
- Animals
- Kinetics
- Rats
- Protein Conformation
- Mutation
- Models, Molecular
- DNA Repair
- Humans