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Article

In vitro evolution driven by epistasis reveals alternative cholesterol-specific binding motifs of perfringolysin O.

The Journal of biological chemistry - 1 Sept 2024

Šakanović Aleksandra, Kranjc Nace, Omersa Neža, Aden Saša, Kežar Andreja, Kisovec Matic, Zavec Apolonija Bedina, Caserman Simon, Gilbert Robert J C, Podobnik Marjetka, Crnković Ana, Anderluh Gregor

Abstract excerpt

The crucial molecular factors that shape the interfaces of lipid-binding proteins with their target ligands and surfaces remain unknown due to the complex makeup of biological membranes. Cholesterol, the major modulator of bilayer structure in mammalian cell membranes, is recognized by various proteins, including the well-studied cholesterol-dependent cytolysins. Here, we use in vitro evolution to investigate the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Clostridium perfringens

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Identifiers

PMID: 39128714
DOI: 10.1016/j.jbc.2024.107664
PMCID: PMC11416283

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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