Article
Bacterial surface display of PETase mutants and MHETase for an efficient dual-enzyme cascade catalysis.
Bioresource technology - 1 Sept 2024
Xue Kai, Bai Zhonghu, Fordour Eric, Guo Siqi, Zhou Yuxuan, Yang Yankun, Liu Xiuxia, Li Ye, Liu Chun-Li
Abstract excerpt
Biological degradation of PET plastic holds great potential for plastic recycling. However, the high costs associated with preparing free enzymes for degrading PET make it unfeasible for industrial applications. Hence, we developed various cell catalysts by surface-displaying PETase mutants and MHETase using autotransporters in E. coli and P. putida. The efficiency of surface display was enhanced through...
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