Article
Protein engineering of stable IsPETase for PET plastic degradation by Premuse.
International journal of biological macromolecules - 1 Jun 2021
Meng Xiangxi, Yang Lixin, Liu Hanqing, Li Qingbin, Xu Guoshun, Zhang Yan, Guan Feifei, Zhang Yuhong, Zhang Wei, Wu Ningfeng, Tian Jian
Abstract excerpt
Poly(ethylene terephthalate) (PET) is used widely by human beings, but is very difficult to degrade. Up to now, the PET degradation effect of PETase from Ideonella sakaiensis 201-F6 (IsPETase) variants with low stability and activity was not ideal. In this study, a mutation design tool, Premuse, was developed to integrate the sequence alignment and quantitative selection of the preferred mutations based on...
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