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Article

Simultaneous Native Mass Spectrometry Analysis of Single and Double Mutants To Probe Lipid Binding to Membrane Proteins.

Analytical chemistry - 25 Jun 2024

Jayasekera Hiruni S, Mohona Farhana Afrin, Ewbank Megan, Marty Michael T

Abstract excerpt

Lipids are critical modulators of membrane protein structure and function. However, it is challenging to investigate the thermodynamics of protein-lipid interactions because lipids can simultaneously bind membrane proteins at different sites with different specificities. Here, we developed a native mass spectrometry (MS) approach using single and double mutants to measure the relative energetic contributions of...

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PMID: 38859611
DOI: 10.1021/acs.analchem.4c01704
PMCID: PMC11215972

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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