Article
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry.
Journal of visualized experiments : JoVE - 14 Oct 2021
Depierreux Delphine M, Seshadri Emily, Shmeleva Evgeniya V, Kieckbusch Jens, Hawkes Delia A, Colucci Francesco
Abstract excerpt
Described here is a simple method to isolate and phenotype mouse group 1 uterine innate lymphoid cells (g1 uILCs) from individual pregnant uterus by flow cytometry. The protocol describes how to set up time mating to obtain multiple synchronous dams, the mechanical and enzymatic digestion of the pregnant uterus, the staining of single-cell suspensions, and a FACS strategy to phenotype and discriminate g1 uILCs....
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