Article
The use of intracellular dyes to create a multiplexed flow cytometry-based red blood cell phenotyping assay.
Vox sanguinis - 1 Jul 2024
Liwski Robert, Greenshields Anna, Grace Ian, Rourke Craig, Cheng Calvino, Quinn Jason George
Abstract excerpt
BACKGROUND AND OBJECTIVES: Flow cytometry can be used to phenotype red blood cell antigens, allowing for high-throughput testing while using low reagent volumes. This article utilizes intracellular dyes to pre-label red blood cells to further multiplex flow cytometry-based red blood cell antigen phenotyping. MATERIALS AND METHODS: Red blood cells were pre-labelled using the intracellular dyes V450 and Oregon...
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