Article
Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Jun 2005
Mittag Anja, Lenz Dominik, Gerstner Andreas O H, Sack Ulrich, Steinbrecher Michael, Koksch Mario, Raffael Alexander, Bocsi Jozsef, Tárnok Attila
Abstract excerpt
BACKGROUND: Natural killer (NK) and NK T (NKT) cells are important in innate immune defense. Their unequivocal identification requires at least four antigens. Based on the expression of additional antigens, they can be further divided into functional subsets. For more accurate immunophenotyping and to describe multiple expression patterns of leukocyte subsets, an increased number of measurable colors is...
Topics
- CD3 Complex
- CD4 Antigens
- CD56 Antigen
- CD8 Antigens
- Carbocyanines
- Flow Cytometry
- Humans
- Immunophenotyping
- Killer Cells, Natural
- Leukocytes
- Lymphocyte Subsets
- Lymphocytes
- Neutrophils