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Article

Measurement of KRAS-GTPase Activity.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Rabara Dana, Stephen Andrew G

Abstract excerpt

Oncogenic mutations in KRAS typically impact the GAP-mediated and intrinsic GTP hydrolysis activity resulting in elevated levels of cellular KRAS-GTP. The development of biochemical assays for GTPase activity provides an opportunity to quantitatively measure the impact of these mutations on GTP hydrolysis. Here we describe a biochemical assay that measures the release of free phosphate upon hydrolysis of the GTP...

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Share to KRAS gene
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Proto-Oncogene Proteins p21(ras)

Source

Identifiers

PMID: 38570454
DOI: 10.1007/978-1-0716-3822-4_7

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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