Article
Pacybara: accurate long-read sequencing for barcoded mutagenized allelic libraries.
Bioinformatics (Oxford, England) - 29 Mar 2024
Weile Jochen, Ferra Gabrielle, Boyle Gabriel, Pendyala Sriram, Amorosi Clara, Yeh Chiann-Ling, Cote Atina G, Kishore Nishka, Tabet Daniel, van Loggerenberg Warren, Rayhan Ashyad, Fowler Douglas M, Dunham Maitreya J, Roth Frederick P
Abstract excerpt
MOTIVATION: Long-read sequencing technologies, an attractive solution for many applications, often suffer from higher error rates. Alignment of multiple reads can improve base-calling accuracy, but some applications, e.g. sequencing mutagenized libraries where multiple distinct clones differ by one or few variants, require the use of barcodes or unique molecular identifiers. Unfortunately, sequencing errors can...
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