Article
Scaffold-Based (Matrigel™) 3D Culture Technique of Glioblastoma Recovers a Patient-like Immunosuppressive Phenotype.
Cells - 14 Jul 2023
Braun Frank K, Rothhammer-Hampl Tanja, Lorenz Julia, Pohl Sandra, Menevse Ayse-Nur, Vollmann-Zwerenz Arabel, Bumes Elisabeth, Büttner Maren, Zoubaa Saida, Proescholdt Martin, Schmidt Nils O, Hau Peter, Beckhove Philipp, Winner Beate, Riemenschneider Markus J
Abstract excerpt
Conventional 2D cultures are commonly used in cancer research though they come with limitations such as the lack of microenvironment or reduced cell heterogeneity. In this study, we investigated in what respect a scaffold-based (Matrigel™) 3D culture technique can ameliorate the limitations of 2D cultures. NGS-based bulk and single-cell sequencing of matched pairs of 2D and 3D models showed an altered...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.