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Development of long InDel markers through whole genome resequencing for variety purity detection in cucumber.

Scientific reports - 13 May 2026

Tian Haoran, Yang Jingjing, Li Qiang, Sui Xuelian, Lv Na, Naveed Shahzad Amir, Wang Yuetian, Zhang Jian, Wang Ying

Abstract excerpt

The purity of commercial cucumber varieties, predominantly F1 hybrids, is a critical factor in seed quality assessment. While SSR or SNP markers are commonly used, insertion-deletion (InDel) markers offer greater stability and practical advantages, including lower cost, compatibility with agarose gel electrophoresis, and suitability for multiplex PCR. In this study, we developed long InDel (> 30 bp) markers for...

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PMID: 42129274
DOI: 10.1038/s41598-026-52824-w
PMCID: PMC13396215

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