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Affinity-enhanced RNA-binding domains as tools to understand RNA recognition.

Cell reports methods - 26 Jun 2023

Chaves-Arquero Belén, Collins Katherine M, Abis Giancarlo, Kelly Geoff, Christodoulou Evangelos, Taylor Ian A, Ramos Andres

Abstract excerpt

Understanding how the RNA-binding domains of a protein regulator are used to recognize its RNA targets is a key problem in RNA biology, but RNA-binding domains with very low affinity do not perform well in the methods currently available to characterize protein-RNA interactions. Here, we propose to use conservative mutations that enhance the affinity of RNA-binding domains to overcome this limitation. As a proof...

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Identifiers

PMID: 37426752
DOI: 10.1016/j.crmeth.2023.100508
PMCID: PMC10326445

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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