Article
KH domains with impaired nucleic acid binding as a tool for functional analysis.
Nucleic acids research - 1 Aug 2012
Hollingworth David, Candel Adela M, Nicastro Giuseppe, Martin Stephen R, Briata Paola, Gherzi Roberto, Ramos Andres
Abstract excerpt
In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these proteins operate. We have designed a KH mutant with impaired RNA-binding capability for general use in...
Topics
- Amino Acid Sequence
- Molecular Sequence Data
- Mutation
- Protein Interaction Domains and Motifs
- RNA
- RNA Stability
- RNA, Messenger
- RNA-Binding Proteins
- Trans-Activators