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Efficient and versatile multiplex prime editing in hexaploid wheat.

Genome biology - 29 Jun 2023

Ni Pei, Zhao Yidi, Zhou Ximeng, Liu Zehua, Huang Zhengwei, Ni Zhongfu, Sun Qixin, Zong Yuan

Abstract excerpt

Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fold and 6.4-fold compared to the original PPE and ePPE, respectively. Importantly, a robust multiplex prime editing platform is...

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PMID: 37386475
DOI: 10.1186/s13059-023-02990-1
PMCID: PMC10308706

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