Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The effect of DNA extraction methodology on gut microbiota research applications

26 Jul 2016

Abstract excerpt

BACKGROUND: The effect that traditional and modern DNA extraction methods have on applications to study the role of gut microbiota in health and disease is a topic of current interest. Genomic DNA was extracted from three faecal samples and one probiotic capsule using three popular methods; chaotropic (CHAO) method, phenol/chloroform (PHEC) extraction, proprietary kit (QIAG). The performance of each of these...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clostridium difficile and Clostridium perfringens research
Share to Fecal contamination and water quality
Share to Gut microbiota and health

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 27456340
DOI: 10.1186/s13104-016-2171-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The effect of DNA extraction methodology on gut microbiota research applicationsPMID 27456340Read ↗

Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiomeSemantic proximity Read ↗

Choice of DNA extraction method affects stool microbiome recovery and subsequent phenotypic association analysesSemantic proximity Read ↗

Evaluation of fecal DNA extraction protocols for human gut microbiome studiesSemantic proximity Read ↗

High-throughput DNA extraction strategy for fecal microbiome studiesSemantic proximity Read ↗

A unique and reliable fecal DNA extraction method for 16S rRNA gene and shotgun metagenomic sequencing in the analysis of the human gut microbiomeSemantic proximity Read ↗

Choice of DNA extraction method affects stool microbiome recovery and subsequent phenotypic association analysesSemantic proximity Read ↗

Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differencesSemantic proximity Read ↗

Stool sampling and DNA isolation kits affect DNA quality and bacterial composition following 16S rRNA gene sequencing using MiSeq Illumina platformSemantic proximity Read ↗

Optimisation of sample storage and DNA extraction for human gut microbiota studiesSemantic proximity Read ↗

The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene SequencingSemantic proximity Read ↗

Optimizing storage, high-molecular weight DNA extraction and genome reconstructions from human faecal samplesSemantic proximity Read ↗

Fecal Microbiota Analysis: An Overview of Sample Collection Methods and Sequencing StrategiesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.