Article
Hi-plex deep amplicon sequencing for identification, high-resolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep.
EBioMedicine - 1 Jul 2023
Jouet Agathe, Braet Sofie Marijke, Gaudin Cyril, Bisch Gaëlle, Vasconcellos Sidra, Epaminondas Nicacio de Oliveira do Livramento Rebecca Emmanuela, Prado Palacios Yrneh Yadamis, Fontes Amanda Brum, Lucena Norma, Rosa Patricia, Moraes Milton, La Kevin, Badalato Nelly, Lenoir Esteban, Ferré Alice, Clément Marie, Hasker Epco, Grillone Silahi Halifa, Abdou Wirdane, Said Aouladi, Assoumani Younoussa, Attoumani Nissad, Laurent Yannick, Cambau Emmanuelle, de Jong Bouke Catherine, Suffys Philip Noël, Supply Philip
Abstract excerpt
BACKGROUND: Expansion of antimicrobial resistance monitoring and epidemiological surveillance are key components of the WHO strategy towards zero leprosy. The inability to grow Mycobacterium leprae in vitro precludes routine phenotypic drug susceptibility testing, and only limited molecular tests are available. We evaluated a culture-free targeted deep sequencing assay, for mycobacterial identification,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.