Article
FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Murray Liam, Olson Meagan N, Barton Nathaniel, Dawes Pepper, Chan Yingleong, Lim Elaine T
Abstract excerpt
Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and...
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