Article
Short Tandem Repeat DNA Profiling Using Perylene-Oligonucleotide Fluorescence Assay.
Analytical chemistry - 23 May 2023
Hernández Bustos Adrián, Martiny Elisa, Bom Pedersen Nadia, Parvathaneni Rohith Pavan, Hansen Jonas, Ji Hanlee P, Astakhova Kira
Abstract excerpt
We report an amplification-free genotyping method to determine the number of human short tandem repeats (STRs). DNA-based STR profiling is a robust method for genetic identification purposes such as forensics and biobanking and for identifying specific molecular subtypes of cancer. STR detection requires polymerase amplification, which introduces errors that obscure the correct genotype. We developed a new method...
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