Article
Rapid PCR of STR markers: Applications to human identification.
Forensic science international. Genetics - 1 Sept 2015
Romsos Erica L, Vallone Peter M
Abstract excerpt
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection, multiplex PCR is commonly identified as the bottleneck in the process. The time requirement of up to three hours to complete 28-30 cycles of multiplex PCR for STR...
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