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Enzyme Cascade with Horseradish Peroxidase Readout for High-Throughput Screening and Engineering of Human Arginase-1.

Analytical chemistry - 9 May 2023

Fernández De Santaella Jaime, Ren Jin, Vanella Rosario, Nash Michael A

Abstract excerpt

We report an enzyme cascade with horseradish peroxidase-based readout for screening human arginase-1 (hArg1) activity. We combined the four enzymes hArg1, ornithine decarboxylase, putrescine oxidase, and horseradish peroxidase in a reaction cascade that generated colorimetric or fluorescent signals in response to hArg1 activity and used this cascade to assay wild-type and variant hArg1 sequences as soluble...

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PMID: 37094096
DOI: 10.1021/acs.analchem.2c05429

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