Article
[A new method for SNP typing based on allele specific PCR].
Fa yi xue za zhi - 1 Jun 2008
Wang Rui-Heng, Liu Li-Min, Zhao Jin-Ling, Sun Xue-Ke, Sun Lin-Lin, Zhou Gang
Abstract excerpt
OBJECTIVE: To establish a new method of SNP typing. METHODS: Based on the principle of allele specific PCR and capillary electrophoresis technique, 11 diallelic SNP loci were selected and two forward primers with different length were designed for each SNP, with their 3' ends matched to the two alleles, respectively. An artificially mismatched base was also introduced into the third or fourth base in the 3' end...
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