Article
Development of Highly Sensitive Digital Droplet PCR for Detection of cKIT Mutations in Circulating Free DNA That Mediate Resistance to TKI Treatment for Gastrointestinal Stromal Tumor (GIST).
International journal of molecular sciences - 12 Mar 2023
Rassner Michael, Waldeck Silvia, Follo Marie, Jilg Stefanie, Philipp Ulrike, Jolic Martina, Wehrle Julius, Jost Philipp J, Peschel Christian, Illert Anna Lena, Duyster Justus, Scherer Florian, von Bubnoff Nikolas
Abstract excerpt
BACKGROUND: Mutations in cKIT or PDGFRA are found in up to 90% of patients with gastrointestinal stromal tumors (GISTs). Previously, we described the design, validation, and clinical performance of a digital droplet (dd)PCR assay panel for the detection of imatinib-sensitive cKIT and PDFGRA mutations in circulating tumor (ct)DNA. In this study, we developed and validated a set of ddPCR assays for the detection of...
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