Article
Evaluation of Radiation Sensitivity Differences in Mouse Liver Tumor Organoids Using CRISPR/Cas9-Mediated Gene Mutation.
Technology in cancer research & treatment - 1 Jan 2000
Jeon Wan, Jung Se Yeon, Lee Chae Young, Kim Won-Tae, Kim Hyun, Jang Kyoung Won, Lim Heuijin, Lee Manwoo, Jeong Dong Hyeok, Kim Sung Dae, Kim In Ah, Choi Si Ho, Son Tae Gen, Kim Kyung Su
Abstract excerpt
BACKGROUND: To assess the radiosensitivity of liver tumors harboring different genetic mutations, mouse liver tumors were generated in vivo through the hydrodynamic injection of clustered regularly interspaced short palindromic repeat/caspase 9 (CRISPR/Cas9) constructs encoding single-guide RNAs (sgRNAs) targeting Tp53, Pten, Nf1, Nf2, Tsc2, Cdkn2a, or Rb1. METHODS: The plasmid vectors were delivered to the liver...
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