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Development of a sensitive double TaqMan Probe-based qPCR Angle-Degree method to detect mutation frequencies.

Mitochondrion - 1 May 2023

Tomita Kazuo, Indo Hiroko P, Sato Tomoaki, Tangpong Jitbanjong, Majima Hideyuki J

Abstract excerpt

We designed a method to examine the mutation frequencies of the A3243G mutation of mitochondrial DNA (mtDNA) in patients with mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome. We performed a qPCR assay using the FAM and VIC TaqMan probes, which detect the 3243G (mutated) and 3243A (wild-type) sequences of mtDNA, respectively. The results obtained by "degree" in a series of...

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PMID: 36841519
DOI: 10.1016/j.mito.2023.02.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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