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[AS-PCR assay for 20 mtDNA SNP typing and haplotype frequency].

Fa yi xue za zhi - 1 Apr 2014

Nie Yan-Chai, Zhang Chen, Liu Ya-Nan, Huang Jiang-Ping, Jiao Hai-Tao, Wu Dan, Zhou Huai-Gu

Abstract excerpt

OBJECTIVE: To develop a multiplex allele-specific PCR (AS-PCR) assay with three-color fluorescence labeling for mitochondrial DNA (mtDNA) SNP typing. METHODS: Based on the principle of AS-PCR, the primer sets were designed for 20 SNP located on the coding region of mtDNA and divided into 2 groups labeled with FAM and HEX fluorescence, respectively. A primer set included two forward (reverse) allelic specific...

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PMID: 25073315

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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