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CRISPR/Cas9 technology in tumor research and drug development application progress and future prospects

8 Jul 2025

Abstract excerpt

The CRISPR/Cas9 system is an acquired immune defense mechanism that has evolved in bacteria and archaea to protect against viral and plasmid attacks. It consists of regularly spaced clusters of short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas). By adapting the simplest type II CRISPR system to utilize special small guide RNA (sgRNA) and Cas9 nucleic acid endonuclease, precise cuts can be...

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PMID: 40697666
DOI: 10.3389/fphar.2025.1552741

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