Article
Overcoming polyploidy pitfalls: a user guide for effective SNP conversion into KASP markers in wheat.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Aug 2020
Makhoul M, Rambla C, Voss-Fels K P, Hickey L T, Snowdon R J, Obermeier C
Abstract excerpt
KEY MESSAGE: Conversion of SNP chip assays into locus-specific KASP markers requires adapted strategies in polyploid species with high genome homeology. Procedures are exemplified by QTL-associated SNPs in hexaploid wheat. Kompetitive allele-specific PCR (KASP) markers are commonly used in marker-assisted commercial plant breeding due to their cost-effectiveness and throughput for high sample volumes. However,...
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