Article
Efficiently Editing Multiple Duplicated Homeologs and Alleles for Recurrent Polyploids.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Gan Rui-Hai, Zhou Li, Gui Jian-Fang
Abstract excerpt
Research on the evolutionary fate of duplicated genes in recurrent polyploids is scarce due to the difficulties in disentangling the different homeologs and alleles of duplicated genes. This chapter describes the detailed procedures to identify different homeologs and alleles of duplicated genes, to analyze their molecular characteristics, and to reveal their functional divergence by gene editing with CRISPR/Cas9...
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