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Thousands of trait-specific KASP markers designed for diverse breeding applications in rice (Oryza sativa).

G3 (Bethesda, Md.) - 8 Jan 2025

Steele Katherine, Quinton-Tulloch Mark, Vyas Darshna, Witcombe John

Abstract excerpt

This study aimed to broaden applicability of KASP for Oryza sativa across diverse genotypes through incorporation of ambiguous (degenerate) bases into their primer designs and to validate 4,000 of them for genotyping applications. A bioinformatics pipeline was used to compare 129 rice genomes from 89 countries with the indica reference genome R498 and generate ∼1.6 million KASP designs for the more common...

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PMID: 39486028
DOI: 10.1093/g3journal/jkae251
PMCID: PMC11708223

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