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Construction of an infectious Chikungunya virus cDNA clone and stable insertion of mCherry reporter genes at two different sites

7 Jun 2012

Abstract excerpt

Chikungunya virus (CHIKV) has caused massive epidemics in the Indian Ocean region since 2005. It belongs to the genus Alphavirus and possesses a positive-stranded RNA genome of nearly 12 kb in size. To produce genetically modified viruses for the study of various aspects of the CHIKV life cycle, a reverse genetic system is needed. We report the generation of a T7 RNA polymerase-driven infectious cDNA clone of...

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PMID: 22673932
DOI: 10.1099/vir.0.043752-0

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