Article
POLθ processes ssDNA gaps and promotes replication fork progression in BRCA1-deficient cells.
Cell reports - 29 Nov 2022
Schrempf Anna, Bernardo Sara, Arasa Verge Emili A, Ramirez Otero Miguel A, Wilson Jordan, Kirchhofer Dominik, Timelthaler Gerald, Ambros Anna M, Kaya Atilla, Wieder Marcus, Ecker Gerhard F, Winter Georg E, Costanzo Vincenzo, Loizou Joanna I
Abstract excerpt
Polymerase theta (POLθ) is an error-prone DNA polymerase whose loss is synthetically lethal in cancer cells bearing breast cancer susceptibility proteins 1 and 2 (BRCA1/2) mutations. To investigate the basis of this genetic interaction, we utilized a small-molecule inhibitor targeting the POLθ polymerase domain. We found that POLθ processes single-stranded DNA (ssDNA) gaps that emerge in the absence of BRCA1,...
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