Article
Engineering of l-threonine aldolase for the preparation of 4-(methylsulfonyl)phenylserine, an important intermediate for the synthesis of florfenicol and thiamphenicol.
Enzyme and microbial technology - 1 Jun 2020
Liu Zhicheng, Chen Xi, Chen Qijia, Feng Jinhui, Wang Min, Wu Qiaqing, Zhu Dunming
Abstract excerpt
l-Threonine aldolases (l-TAs) catalyze the aldol condensation of aldehyde and glycine, offering direct enzymatic synthesis of β-hydroxy-α-amino acids under mild conditions. However, this method suffers from moderate yield and low stereoselectivity at the β-carbon. Given the importance of 4-(methylsulfonyl)phenylserine for the synthesis of florfenicol and thiamphenicol, mutations of a l-threonine aldolase from...
Topics
- Catalysis
- Glycine Hydroxymethyltransferase
- Mutation
- Protein Engineering
- Pseudomonas
- Serine
- Substrate Specificity
- Thiamphenicol