Article
A reconstituted depolarization-induced Ca2+ release platform for validation of skeletal muscle disease mutations and drug discovery.
The Journal of general physiology - 5 Dec 2022
Murayama Takashi, Kurebayashi Nagomi, Numaga-Tomita Takuro, Kobayashi Takuya, Okazaki Satoru, Yamashiro Kyosuke, Nakada Tsutomu, Mori Shuichi, Ishida Ryosuke, Kagechika Hiroyuki, Yamada Mitsuhiko, Sakurai Takashi
Abstract excerpt
In skeletal muscle excitation-contraction (E-C) coupling, depolarization of the plasma membrane triggers Ca2+ release from the sarcoplasmic reticulum (SR), referred to as depolarization-induced Ca2+ release (DICR). DICR occurs through the type 1 ryanodine receptor (RyR1), which physically interacts with the dihydropyridine receptor Cav1.1 subunit in specific machinery formed with additional essential components...
Topics
- Humans
- Ryanodine Receptor Calcium Release Channel
- Calcium
- HEK293 Cells
- Sarcoplasmic Reticulum
- Muscle Fibers, Skeletal
- Calcium Channels, L-Type
- Muscular Diseases
- Muscle, Skeletal
- Mutation
- Drug Discovery