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Efficient generation of mNeonGreen Plasmodium falciparum reporter lines enables quantitative fitness analysis

16 Sept 2022

Abstract excerpt

CRISPR editing has enabled the rapid creation of fluorescent Plasmodium transgenic lines, facilitating a deeper understanding of parasite biology. The impact of genetic perturbations such as gene disruption or the introduction of drug resistance alleles on parasite fitness is typically quantified in competitive growth assays between the query line and a wild type reference. Although fluorescent reporter lines...

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Identifiers

PMID: 36189342
DOI: 10.3389/fcimb.2022.981432

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