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Article

Green Fluorescent Protein as a Marker in Plasmodium berghei Transformation

1 May 1999

Abstract excerpt

We present a new marker that confers both resistance to pyrimethamine and green fluorescent protein-based fluorescence on the malarial parasite Plasmodium berghei. A single copy of the cassette integrated into the genome is sufficient to direct fluorescence in parasites throughout the life cycle, in both its mosquito and vertebrate hosts. Erythrocyte stages of the parasite that express the marker can be sorted...

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PMID: 10225926
DOI: 10.1128/iai.67.5.2602-2606.1999

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